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[ Sigma ] Trypsin-EDTA solution

[ Sigma ] Trypsin-EDTA solution

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Item details
SKU: T4049-100ML

Stock: Pre order

Brand: Sigma - Aldrich
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0.25%, sterile-filtered, BioReagent, suitable for cell culture, 2.5 g porcine trypsin and 0.2 g EDTA • 4Na per liter of Hanks′ Balanced Salt Solution with phenol red


The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture.

Trypsin-EDTA solution was used:
• in detaching HT29 human colorectal cancer cells cultured in RPMI 1640 which was supplemented with 10 % fetal calf serum, during relative cell frequency determination of high concentration samples.[1]
• to trypsinize the transient transfected human embryonic kidney tcA-201 cell line.[2]
• to enzymatically release mouse fibroblasts cells (cell line L929) adhered to the scaffold, during cell culturing to assess the influence of several modified treatments of Poly(L/D)lactide 96/4 non-woven scaffolds and fibres.[3]

biological source   Porcine
sterility   sterile-filtered
product line   BioReagent
form   solution
mol wt   23.4 kDa
concentration   0.25%
application(s)   cell culture | mammalian: suitable
impurities   Porcine parvovirus, none detected (9 CFR)
pH   7.0-7.6
shipped in   dry ice
storage temp.   −20°C

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