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[Sigma] Dulbecco’s Modified Eagle’s Medium - high glucose, 500ML
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[Sigma] Dulbecco’s Modified Eagle’s Medium - high glucose, 500ML

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RM148.00  
Item details
SKU: D6429-500ML

Stock: Pre order

Brand: Sigma - Aldrich
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General description

This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.

Application

Dulbecco’s Modified Eagle’s Medium - high glucose has been used for cell culture.[2][3][1]

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Quality Level   300
sterility   sterile-filtered
form   liquid
application(s)   cell culture | mammalian: suitable
impurities   endotoxin, tested
components   glucose: high
sodium pyruvate: yes
HEPES: no
NaHCO3: yes
L-glutamine: yes
phenol red: yes
shipped in   ambient
storage temp.   2-8°C

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