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[Sigma] Thiazolyl Blue Tetrazolium Bromide

[Sigma] Thiazolyl Blue Tetrazolium Bromide

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Item details
SKU: M5655-500MG

Stock: Pre order

Brand: Sigma - Aldrich
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powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)


Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.

Thiazolyl Blue Tetrazolium Bromide has been used as a colorimetric metabolic activity indicator in cell viability assays (MTT assay).[5][1][2][3] It has also been used to determine cell proliferation.[4]

Quality Level   200
product line   BioReagent
assay   ≥97.5% (HPLC)
form   powder
application(s)   cell culture | insect: suitable
  cell culture | mammalian: suitable
mp   195 °C (dec.) (lit.)
solubility   H2O: 5 mg/mL
storage temp.   2-8°C
SMILES string   [Br-].Cc1nc(sc1C)-[n+]2nc(nn2-c3ccccc3)-c4ccccc4
InChI   1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1

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